The Human Fetal Glial Cell Line SVG p12 Contains Infectious BK Polyomavirus

The human fetal glial cell line SVG p12 contains infectious BK polyomavirus.

UNLABELLED The human fetal glial cell line SVG was generated in 1985 by transfecting primary fetal brain cells with a plasmid containing an origin-defective mutant of simian virus 40 (SV40). The cells, which express SV40 large T-antigen, support the replication of human JC polyomavirus (JCPyV) and have been used for JCPyV studies but also for other studies in which cells of neural origin were d...

The human fetal glial cell line SVG p 12 contains infectious

Monitoring subtypes of the human polyomavirus BK in Iranian adult kidney transplant patients

BK virus (BKV) is a polyomavirus with seroprevalence in adults, ranging from 60 to 100%. It is considered as usual cause of renal dysfunction after the allograft renal transplantation nephropathy. Potent immunosuppressive therapy in kidney transplantation can lower the rate of acute rejection. Therefore, untreated BKV infections lead to kidney allograft dysfunction or loss. In order to estimate...

The agnogene of the human polyomavirus BK is expressed.

Primate polyomavirus genomes all contain an open reading frame at the 5' end of the late coding region called the agnogene. A simian virus 40 agnoprotein with unknown functions has previously been demonstrated. We now show that a BK virus agnoprotein appears in the perinuclear area and cytoplasm late in the infectious cycle. It is phosphorylated in vivo and coimmunoprecipitates with a subset of...

Human BK Polyomavirus Plasmid pBKV (34-2) (Dunlop) Contains Mutations Not Found in the Originally Published Sequences

The plasmid pBKV (34-2) (ATCC 45025) contains the entire BK polyomavirus Dunlop genome. Sequencing revealed 12 point mutations compared to the GenBank sequence, but only 4 point mutations compared to the published sequence. The origin of these differences is unknown, but may impact virological as well as diagnostic research and development.